Cloned (Comment) | Organism |
---|---|
gene ompT, phage display metod used for substrate specificity analysis, expression in Escherichia coli AN1 cells | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics for phage display generated peptide substrates, overview | Escherichia coli K-12 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Escherichia coli K-12 | 19867 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
human plasminogen + H2O | Escherichia coli K-12 | proteolytic cleavage site CPGR*VVGGC, activation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
gene ompT | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli AN1 cells | Escherichia coli K-12 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val. | the enzyme contains a Ser-Asp-His catalytic triad, active site, the consensus sequence of OmpT is R/K*-R/K, the enzyme is highly selective for a basic amino acid residue at position P1, but less exclusive at P1', where several amino acids are tolerated e,g, Lys, Val, and Gly, at P2' common residues are Ala or Val, at P3 and P4 the enzyme prefers Trp or Arg | Escherichia coli K-12 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-neoendorphin + H2O | proteolytic cleavage site GFLR*KYPK | Escherichia coli K-12 | ? | - |
? | |
dynorphin A(1-13) + H2O | proteolytic cleavage site GFLR*RIRPK | Escherichia coli K-12 | Tyr-Gly-Gly-Phe-Leu-Arg + Arg-Ile-Arg-Pro-Lys-Leu-Lys | - |
? | |
gamma-interferon + H2O | proteolytic cleavage sites KTGK*RKRSQ and FRGR*RASQ | Escherichia coli K-12 | ? | - |
? | |
human creatin kinase + H2O | proteolytic cleavage site DIYK*KLRDK | Escherichia coli K-12 | ? | - |
? | |
human plasminogen + H2O | proteolytic cleavage site CPGR*VVGGC, activation | Escherichia coli K-12 | ? | - |
? | |
Mastoparan + H2O | proteolytic cleavage site ALAK*KIL | Escherichia coli K-12 | ? | - |
? | |
additional information | substrate specificity analysis | Escherichia coli K-12 | ? | - |
? | |
Parathyroid hormone + H2O | proteolytic cleavage sites EWLR*KKLQD and WLRK*KLQDV | Escherichia coli K-12 | ? | - |
? | |
rabbit creatine kinase + H2O | proteolytic cleavage sites DLYK*KLRDK and RGER*RAVEK | Escherichia coli K-12 | ? | - |
? | |
T7 RNA polymerase + H2O | proteolytic cleavage sites QLNK*RVGHV, HVYK*KAFMQ, and DRAR*KSRRI | Escherichia coli K-12 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ompT | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli K-12 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli K-12 |